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Image Search Results
Journal: Hepatology (Baltimore, Md.)
Article Title: Dynamic expression profiling of type I and type III interferon-stimulated hepatocytes reveals a stable hierarchy of gene expression
doi: 10.1002/hep.26657
Figure Lengend Snippet: Huh7 cells were treated with either IFN-α, IFN-β (500 U/ml), or IFN-λ1, IFN-λ2, IFN-λ3 (10 ng/ml), and gene expression was measured at 0, 0.5, 1, 2, 4, 6, 12, and 24 hours post stimulation. a) A total of 434 probesets (rows) were found to be significantly differentially-expressed for at least one time point by at least one IFN (columns). Hierarchical clustering identified six separate groups (numbered boxes). b) The number of genes significantly induced or suppressed by individual IFNs at each time point. c) Comparison of log2 expression values for genes induced after 12 hours of stimulation with IFN-λ1, IFN-λ2 or IFN-λ3. Equivalent expression is indicated by the 1-to-1 line (dotted line), while the solid line shows the line of best fit with slope set to 1.
Article Snippet: Recombinant human IL-29 (IFN-λ1), IL-28A (IFN-λ2), and
Techniques: Expressing, Comparison
Journal: Hepatology (Baltimore, Md.)
Article Title: Dynamic expression profiling of type I and type III interferon-stimulated hepatocytes reveals a stable hierarchy of gene expression
doi: 10.1002/hep.26657
Figure Lengend Snippet: a) Huh7 cells were treated with IFN-α, IFN-β (500 U/ml), IFN-λ1, IFN-λ2, or IFN-λ3 (10 ng/ml), and gene expression of six ISGs from groups 4, 5, and 6 were quantified using RT-qPCR (bottom), and compared to fold-changes from the microarray data (top). Huh7 cells were treated with 10, 100, 500, or 2500 U/ml of IFN-α or IFN-β (b), or with 1 or 10 ng/ml of IFN-λ1, IFN-λ2, or IFN-λ3 (c) at fixed time points, and mRNA expression of MX1, IRF9, IFIT3 and CXCL10 were quantified by RT-qPCR.
Article Snippet: Recombinant human IL-29 (IFN-λ1), IL-28A (IFN-λ2), and
Techniques: Expressing, Quantitative RT-PCR, Microarray
Journal: Hepatology (Baltimore, Md.)
Article Title: Dynamic expression profiling of type I and type III interferon-stimulated hepatocytes reveals a stable hierarchy of gene expression
doi: 10.1002/hep.26657
Figure Lengend Snippet: Primary human hepatocytes were seeded into collagen-coated 12-well plates before treatment with IFN-α, IFN-β (500 U/ml), IFN-λ1, IFN-λ2, or IFN-λ3 (10 ng/ml) for 0, 1, 4, 6, 12, or 24 hours. The expression of six ISGs from groups 4, 5, and 6 were quantified using RT-qPCR.
Article Snippet: Recombinant human IL-29 (IFN-λ1), IL-28A (IFN-λ2), and
Techniques: Expressing, Quantitative RT-PCR